Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

481
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
481
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

475
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
475
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

1.4K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alteration history of aluminum-rich rocks at Jezero crater, Mars.

Communications earth & environment·2025
Same author

Advanced analytical solutions for large-scale works of art: Revealing damage in the renaissance wall paintings of Valencia cathedral (Spain).

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

Intense alteration on early Mars revealed by high-aluminum rocks at Jezero crater.

Communications earth & environment·2024
Same author

Radiation-induced alteration of apatite on the surface of Mars: first in situ observations with SuperCam Raman onboard Perseverance.

Scientific reports·2024
Same author

Geographical distribution of metals and metalloids along the estuary of the Oka River in the biosphere reserve of Urdaibai, Spain.

Marine pollution bulletin·2024
Same author

Multi-analytical characterization of an oncoid from a high altitude hypersaline lake using techniques employed in the Mars2020 and Rosalind Franklin missions on Mars.

Analytica chimica acta·2023

Related Experiment Video

Updated: Jul 30, 2025

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

7.2K

A new methodology for kerogen maturity estimation based on Raman spectroscopy and chemometric analysis.

J Aramendia1, M Tuite2, K Castro1

  • 1Department of Analytical Chemistry, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, Spain.

The Science of the Total Environment
|May 13, 2023
PubMed
Summary

This study introduces a new chemometric method using principal component analysis (PCA) to assess carbonaceous matter maturity from Raman spectra. This approach avoids complex band decomposition, reducing uncertainty in maturity and H:C ratio comparisons.

Keywords:
ChemometricsH:C ratioKerogenNon-destructiveRaman spectroscopy

More Related Videos

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
07:51

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Published on: June 10, 2017

12.0K
Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

18.4K

Related Experiment Videos

Last Updated: Jul 30, 2025

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

7.2K
Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
07:51

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Published on: June 10, 2017

12.0K
Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

18.4K

Area of Science:

  • Geochemistry
  • Materials Science
  • Spectroscopy

Background:

  • Raman spectroscopy is used to determine carbonaceous matter (CM) maturity in geologic samples.
  • Current methods rely on mathematical decomposition of Raman bands, introducing uncertainty due to variations in methods, software, and user interpretation.
  • Spectra pre-treatment and spectrum-by-spectrum analysis can lead to bias and wide uncertainty ranges.

Purpose of the Study:

  • To propose an alternative chemometric method for assessing CM maturity using Raman spectroscopy.
  • To overcome the limitations of traditional methods, such as mathematical band decomposition and the need for spectra pre-treatment.
  • To provide a more robust and less biased approach for comparing CM maturity and H:C ratios.

Main Methods:

  • Utilized principal component analysis (PCA) on the entire spectral range of Raman data.
  • Applied a chemometric approach that considers the whole spectrum, rather than specific regions or band decompositions.
  • Avoided the requirement for spectra pre-treatment, simplifying the analysis process.

Main Results:

  • The proposed PCA method effectively groups coal standard samples by maturity.
  • The method allows for comparative analysis of different carbonaceous matter samples regarding their maturity and H:C ratio.
  • Demonstrated that considering the entire spectrum circumvents uncertainties associated with band decomposition techniques.

Conclusions:

  • The developed chemometric method offers a reliable alternative for assessing carbonaceous matter maturity using Raman spectroscopy.
  • Principal component analysis (PCA) provides a robust framework for comparative maturity assessments without complex spectral manipulation.
  • This approach minimizes user-induced bias and uncertainty, enhancing the reliability of geochemical maturity studies.