Excitation-Emission Matrix Fluorescence Spectroscopy Coupled with PARAFAC Modeling for Viability Prediction of Cells

Area of Science:

• Biotechnology
• Analytical Chemistry
• Cell Biology

Background:

• Cell viability assays are crucial for drug discovery and bioanalysis.
• Traditional methods like MTT, XTT, and LDH assays have limitations including time consumption, labor intensity, and lack of real-time monitoring.
• Existing assays often fail to provide continuous, non-destructive observation of cellular changes.

Purpose of the Study:

• To develop and validate a novel, non-invasive cell viability testing method.
• To offer a faster, more sensitive, and cost-effective alternative to current gold-standard assays.
• To enable real-time, continuous, and non-destructive monitoring of cell viability.

Main Methods:

• Utilized native excitation-emission matrix fluorescence spectroscopy.
• Applied parallel factor analysis (PARAFAC) for data analysis.
• Validated the method on A375 and HaCaT cells treated with oxaliplatin.

Main Results:

• The proposed fluorescence spectroscopy and PARAFAC method demonstrated high accuracy and sensitivity, outperforming the MTT assay.
• The approach allows for non-invasive, non-destructive, and real-time monitoring of cell viability.
• PARAFAC analysis enabled the study of mechanisms behind cell viability changes by tracking fluorophore variations.

Conclusions:

• Native excitation-emission matrix fluorescence spectroscopy coupled with PARAFAC is a powerful tool for cell viability assessment.
• This method provides a reliable and sensitive alternative for real-time cell monitoring in drug discovery and research.
• The technique facilitates a deeper understanding of cellular responses to treatments.