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Updated: Sep 21, 2026
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
Protocol for preparation of a photo-triggering double cross-linked adhesive, antibacterial, and biocompatible
Xinrang Zhai1, Miner Hu2, Honghua Hu2
1International Institutes of Medicine, the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China; School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China.
Abstract:
The treatment of full-thickness skin wounds represents a major clinical challenge, and hydrogel is regarded as a promising class of biomaterials for wound repair. Here, we present a protocol for preparing a photo-triggering double cross-linked, adhesive, antibacterial, and biocompatible hydrogel. We describe the steps to prepare the hydrogel and evaluate its mechanical performance, swelling kinetics, antibacterial property, biocompatibility in vitro, and therapeutic effect in vivo. This protocol is also applicable to other defect models of wound injury. For complete details on the use and execution of this protocol, please refer to our previous work.1.
