Thermodynamic sensitivity of ammonia oxidizers-driven N₂O fluxes under oxic-suboxic realms

In terrestrial ecosystems, the nitrogen dynamics, including N₂O production, are majorly regulated by a complex consortium of microbes favored by different substrates and environmental conditions. To better predict the daily, seasonal and annual variation in N₂O fluxes, it is critical to estimate the temperature sensitivity of different microbial groups for N₂O fluxes under oxic and suboxic conditions prevalent in soil and wetlands. Here, we studied the temperature sensitivity of two groups of ammonia oxidizers, archaea (AOA) and bacteria (AOB), in relation to N₂O fluxes through both nitrification and nitrifier-denitrification pathways across a wide temperature gradient (10-55 °C). Using square root theory (SQRT) and macromolecular rate theory (MMRT) models, we estimated thermodynamic parameters and cardinal temperatures, including maximum temperature sensitivity (T_Smax). The distinction between N₂O pathways was facilitated by microbial-specific inhibitors (PTIO and C₂H₂) and controlled oxygen supply environments (oxic: ambient level; and suboxic: ∼4%). We found that nitrification supported by AOA (Nt_A) and AOB (Nt_B) dominated N₂O production in an oxic climate, while only AOB-supported nitrifier-denitrification (ND_B) majorly contributed (>90%) to suboxic N₂O budget. The models predicted significantly higher optimum temperature (T_opt) and T_Smax for Nt_A and ND_B compared to Nt_B. Intriguingly, both Nt_B and ND_B exhibited significantly wider temperature ranges than Nt_A. Altogether, our results suggest that temperature and oxygen supply control the dominance of specific AOA- and AOB-supported N₂O pathways in soil and sediments. This emergent understanding can potentially contribute toward novel targeted N₂O inhibitors for GHG mitigation under global warming.