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The Function and Mechanism of Long Non-Coding RNA RP11-23J9.4 in Thyroid Cancer

Lili Zhong1, Xiangfu Ding2, Xiaoliang Xiong3

  • 1Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, The Second Hospital of Jilin University, Changchun, 130041, P.R. China.

Critical Reviews in Eukaryotic Gene Expression
|May 15, 2023
PubMed
Abstract

Insights

Downregulating long non-coding RNA RP11 23J9.4 shows promise for thyroid cancer (TC) treatment. Combined with X-ray radiation, it exhibits a synergistic inhibitory effect on TC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy

Background:

  • Thyroid cancer (TC) remains a significant health concern.
  • Identifying novel therapeutic targets is crucial for improving TC treatment outcomes.
  • Long non-coding RNAs (lncRNAs) are emerging as key regulators in various cancers.

Purpose of the Study:

  • To investigate the potential of lncRNA RP11 23J9.4 as a targeted marker for thyroid cancer therapy.
  • To evaluate the combined effect of lncRNA RP11 23J9.4 downregulation and X-ray radiation on TC.

Main Methods:

  • Downregulation of lncRNA RP11 23J9.4 in papillary thyroid carcinoma (PTC) cells via transfection.
  • Assessment of cellular effects including proliferation, invasion, apoptosis, and cell cycle.
  • Irradiation of transfected cells with 2 Gy X-ray.
  • Analysis of Axin2 gene and protein expression using real-time PCR, Western blotting, and immunohistochemistry.

Main Results:

  • Downregulation of lncRNA RP11 23J9.4 inhibited TC development, partly mediated through Axin2.
  • A synergistic inhibitory effect was observed when lncRNA RP11 23J9.4 downregulation was combined with X-ray radiation.
  • The combination therapy demonstrated significant efficacy against TC.

Conclusions:

  • lncRNA RP11 23J9.4 downregulation, in conjunction with X-ray radiation, presents a synergistic therapeutic strategy for TC.
  • lncRNA RP11 23J9.4 serves as a potential biomarker for targeted therapy in thyroid cancer.

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