Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in crypt cell DNA content during experimental colonic carcinogenesis.

J Matthews, T Cooke

    British Journal of Cancer
    |June 1, 1986
    PubMed
    Summary

    During experimental carcinogenesis, colonic crypt cells showed increased DNA content, even in histologically normal tissue. This suggests more dividing cells in colonic crypts during tumor development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The GEMstone Maze: Gamification of Medical Skills and Knowledge.

    The clinical teacher·2026
    Same author

    Energy Spectrum of Ultrahigh-Energy Cosmic Rays across Declinations -90° to +44.8° as Measured at the Pierre Auger Observatory.

    Physical review letters·2026
    Same author

    Search for Light Dark Matter with NEWS-G at the Laboratoire Souterrain de Modane Using a Methane Target.

    Physical review letters·2025
    Same author

    Search for the Anomalous Events Detected by ANITA Using the Pierre Auger Observatory.

    Physical review letters·2025
    Same author

    Inference of the Mass Composition of Cosmic Rays with Energies from 10^{18.5} to 10^{20}  eV Using the Pierre Auger Observatory and Deep Learning.

    Physical review letters·2025
    Same author

    Demonstrating Agreement between Radio and Fluorescence Measurements of the Depth of Maximum of Extensive Air Showers at the Pierre Auger Observatory.

    Physical review letters·2024

    Area of Science:

    • Oncology
    • Cell Biology
    • Gastroenterology

    Background:

    • Colorectal cancer development involves complex cellular changes.
    • Understanding early molecular alterations in colonic crypts is crucial for cancer research.

    Purpose of the Study:

    • To investigate changes in colonic crypt cell DNA content during experimental carcinogenesis.
    • To correlate DNA content alterations with the progression of colorectal tumors in a rat model.

    Main Methods:

    • Experimental carcinogenesis induced in Wistar rats using azoxymethane.
    • Feulgen reaction staining for DNA content analysis in colonic tissues.
    • Microdensitometry used to quantify DNA content in proliferative and functional cells.

    Main Results:

    • Increased mean DNA content observed in distal colon proliferative and functional cells as carcinogenesis progressed.
    • Transitional mucosa adjacent to tumors exhibited similar DNA content increases.
    • Adenomas showed a further elevation in functional cell DNA content.

    Conclusions:

    • Elevated DNA content in colonic crypt cells indicates an increase in dividing cells during carcinogenesis.
    • These findings highlight early cellular changes preceding visible tumor formation.
    • The study provides insights into the molecular mechanisms of colorectal cancer initiation.

    Related Experiment Videos