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Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
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Nightshift work can induce oxidative DNA damage: a pilot study.

Yutong Zou1, Xiaoli Ma1,2, Qian Chen1

  • 1Department of Laboratory Medicine, Peking Union Medical College, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1 Shuaifu Yuan, Dongcheng District, Beijing, 100730, PR China.

BMC Public Health
|May 15, 2023
PubMed
Summary
This summary is machine-generated.

Night shift work increases oxidative DNA damage, indicated by elevated 8-oxodG levels, persisting even a month after cessation. This DNA damage is linked to altered metabolic biomarkers, highlighting potential long-term health risks.

Keywords:
8-oxo-7,8-dihydro-2'-deoxyguanosine8-oxo-7,8-dihydroguanosineNight shiftNucleic-acid damage

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Occupational Health

Background:

  • Regular sleep is crucial for human health.
  • The metabolic effects of night shift work, including oxidative stress and DNA damage, remain under-evaluated in realistic cohort studies.
  • This study addresses the need for long-term follow-up to assess night shift impacts on DNA damage.

Purpose of the Study:

  • To evaluate the effect of night shift work on DNA damage using a long-term follow-up cohort study.
  • To investigate changes in nucleic acid damage markers during and after night shift periods.
  • To explore associations between DNA damage markers and routine metabolic biomarkers.

Main Methods:

  • Recruited 16 healthy night shift workers and collected serum and urine samples at four time points.
  • Measured levels of 8-oxo-7,8-dihydroguanosine (8-oxoG) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) using LC-MS/MS.
  • Employed Mann-Whitney U or Kruskal-Wallis tests for comparisons and correlation analyses.

Main Results:

  • Serum 8-oxodG levels and the serum-to-urine 8-oxodG ratio significantly increased during night shifts.
  • Elevated 8-oxodG levels persisted for at least 1 month after discontinuing night shifts.
  • 8-oxoG and 8-oxodG levels showed significant positive associations with total bilirubin and urea, and negative associations with total cholesterol.

Conclusions:

  • Night shift work may elevate oxidative DNA damage, with effects lasting beyond the work period.
  • Further large-scale studies are needed to clarify short- and long-term effects and develop mitigation strategies.
  • Findings suggest potential long-term health implications of night shift work on DNA integrity.