Related Experiment Video
Updated: Jul 31, 2026
08:28
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
Published on: June 30, 2017
9.5K
CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p
Chunli Hu1, Xue Feng1, Yanfen Ma1
1Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Cells
|May 16, 2023
Summary
Circular RNAs (circRNAs) regulate bovine fat deposition by targeting miR-10167-3p. CircADAMTS16 inhibits adipocyte differentiation and promotes proliferation, impacting beef quality.
Area of Science:
- Molecular Biology
- Animal Science
- Genetics
Background:
- Circular RNAs (circRNAs) are non-coding RNA molecules involved in eukaryotic gene regulation.
- circRNAs are implicated in bovine fat deposition, but their specific roles and mechanisms are not fully understood.
- circADAMTS16, derived from the ADAMTS16 gene, is highly expressed in bovine adipose tissue, suggesting a role in lipid metabolism.
Purpose of the Study:
- To investigate the regulatory mechanism of circADAMTS16 in bovine adipocytes.
- To elucidate the interaction between circADAMTS16 and miR-10167-3p.
- To determine the effects of circADAMTS16 and miR-10167-3p on adipocyte differentiation, proliferation, and apoptosis.
Main Methods:
- Dual-luciferase reporter assay to confirm the targeting relationship between circADAMTS16 and miR-10167-3p.
- Gain-of-function and loss-of-function experiments in bovine adipocytes.
- Real-time quantitative PCR (qPCR) for gene expression analysis.
- Oil Red O staining for lipid droplet assessment.
- CCK-8, EdU assays, and flow cytometry for cell proliferation and apoptosis analysis.
Main Results:
- circADAMTS16 directly targets and binds to miR-10167-3p.
- Overexpression of circADAMTS16 inhibited bovine adipocyte differentiation, while miR-10167-3p overexpression promoted it.
- circADAMTS16 promoted adipocyte proliferation by facilitating cell cycle progression (G0/G1 to S phase) and inhibiting apoptosis.
- Conversely, miR-10167-3p overexpression inhibited proliferation and induced apoptosis in bovine adipocytes.
Conclusions:
- circADAMTS16 plays a crucial role in bovine fat deposition by inhibiting adipocyte differentiation and promoting proliferation through its interaction with miR-10167-3p.
- This study provides novel insights into the regulatory mechanisms of circRNAs in bovine lipid metabolism and beef quality.
