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Circ_0069094 regulates malignant phenotype and paclitaxel resistance in breast cancer cells via targeting the

Zhihua Kong1, Qi Han2, Bisheng Zhu2

  • 1Department of Ultrasound, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, Xianning City, China.

Thoracic Cancer
|May 16, 2023
PubMed
Abstract

Insights

Silencing circ_0069094 enhances paclitaxel sensitivity in breast cancer by sponging miR-136-5p. This circRNA pathway impacts tumor progression and chemoresistance in breast cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of cancer mortality.
  • Circular RNAs (circRNAs) are implicated in cancer progression, often by interacting with microRNAs (miRNAs).
  • The specific role of circ_0069094 in breast cancer remains largely unknown.

Purpose of the Study:

  • To investigate the molecular mechanism of circ_0069094 in breast cancer progression.
  • To elucidate the role of the circ_0069094/miR-136-5p/YWHAZ pathway in malignant behaviors of breast cancer.
  • To assess the impact of circ_0069094 on paclitaxel (PTX) resistance.

Main Methods:

  • Quantitative real-time PCR and Western blot to measure circRNA, miRNA, and mRNA expression.
  • Cell proliferation, invasion, apoptosis, and chemo-sensitivity assays (CCK-8, colony formation, EdU, flow cytometry, transwell).
  • Dual-luciferase reporter assays and xenograft experiments to confirm molecular interactions and in vivo effects.

Main Results:

  • Circ_0069094 was upregulated in PTX-resistant breast cancer tissues and cells.
  • Silencing circ_0069094 inhibited proliferation and invasion, increased apoptosis, and enhanced PTX sensitivity.
  • Circ_0069094 acts as a sponge for miR-136-5p, which targets YWHAZ, thus regulating breast cancer progression.

Conclusions:

  • Circ_0069094 silencing improves PTX sensitivity in breast cancer progression.
  • The circ_0069094/miR-136-5p/YWHAZ axis is a key regulator of breast cancer malignancy and chemoresistance.
  • Targeting circ_0069094 may represent a therapeutic strategy for PTX-resistant breast cancer.
Keywords:
YWHAZbreast cancercirc_0069094miR-136-5ppaclitaxel (PTX)

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