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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Structure, antioxidant properties, and protective effects on DNA damage of exopolysaccharides from Clostridium
Guolin Cai1,2,3, Yongzhe Geng2, Yifan Liu2
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Abstract:
Exopolysaccharides (EPSs) of probiotics are naturally nontoxic antioxidants with some interesting biological activities. This research aims to explore the structural and antioxidant properties of the EPS from Clostridium butyricum, a probiotics widely existed in human and animal intestines. EPS of C. butyricum RO-07 was purified through a combination of anion-exchange column chromatography and gel chromatography and determined to be composed of glucosamine, arabinose, galactosamine, galactose, glucose, and xylose in a molar ratio of 1:1:1:2:1:1 with a molecular weight 1.23 × 104 Da. It exhibited a stronger antioxidant activity than ascorbic acid, with scavenging activities up to 75.2% and 95.0% against hydroxyl radical (•OH) and superoxide radical (O2 - •), respectively. It also performed protective effects on DNA against radiation destruction by ultraviolet and reactive oxygen species generated oxidation stress. With these superior advantages in oxidants and radiation resistance, the EPS from C. butyricum RO-07 therefore has great potential to be applied in food and cosmetic industry.
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