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Related Concept Videos

Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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Electrical Detection Assay Based on Programmable Nucleic Acid Probe for Efficient Single-Nucleotide Polymorphism

Yun Zhang1, Bo Chen1, Duo Chen1

  • 1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Institute of Molecular Medicine, Renmin Hospital of Wuhan University, School of Microelectronics, Wuhan University, Wuhan 430072, P. R. China.

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|May 17, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid, sensitive electrical biosensor for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. The novel assay identifies single-nucleotide mutations, enabling accurate variant discrimination for pandemic screening.

Keywords:
DNA probesRNA mutationsbiosensorsnanodevicessingle-nucleotide polymorphism

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Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Molecular Diagnostics

Background:

  • The COVID-19 pandemic necessitates rapid, on-site detection of SARS-CoV-2 variants.
  • Existing methods often lack the sensitivity and speed for real-time variant identification.
  • The rapid evolution of SARS-CoV-2 demands advanced diagnostic tools capable of single-nucleotide polymorphism (SNP) detection.

Purpose of the Study:

  • To develop a highly sensitive and specific multiplexed electrical detection assay for SARS-CoV-2 variants.
  • To enable on-site nucleic acid testing with single-nucleotide resolution.
  • To create an efficient tool for scalable pandemic screening and variant discrimination.

Main Methods:

  • Utilized a field-effect transistor (FET) biosensor functionalized with a paperclip-shaped nucleic acid probe (PNprobe).
  • Engineered the PNprobe's three-stem structure to amplify thermodynamic stability differences in variant RNAs.
  • Employed combinatorial FET detection channels for simultaneous detection and identification of mutations in seven SARS-CoV-2 variants.

Main Results:

  • Achieved highly sensitive and specific detection and discrimination of SARS-CoV-2 variants.
  • Demonstrated the ability to identify single-nucleotide mutations, including substitutions and deletions, within 15 minutes.
  • Attained an identification accuracy of 97.1% for discriminating SARS-CoV-2 variants in simulated throat swab samples.

Conclusions:

  • The developed multiplexed electrical detection assay offers an efficient method for on-site SARS-CoV-2 variant screening.
  • The biosensor's SNP identification capability is crucial for tracking viral evolution and managing pandemics.
  • This technology provides a scalable solution for rapid molecular diagnostics and public health surveillance.