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Developing a Method for Quantifying Meropenem in Children-Volumetric Adsorptive Microsampling Versus Plasma Sampling.

Ola Ramadan1, Lea Marie Schatz1, Ingeborg van den Heuvel2

  • 1Department of Pharmaceutical and Medical Chemistry, Clinical Pharmacy, University of Münster, Münster, Germany.

Therapeutic Drug Monitoring
|May 18, 2023
PubMed
Summary
This summary is machine-generated.

Volumetric absorptive microsampling (VAMS) is not reliable for meropenem therapeutic drug monitoring in children. A new validated method using 50 µL of plasma provides an effective alternative for pediatric patients.

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Area of Science:

  • Pharmacology
  • Clinical Chemistry
  • Pediatric Critical Care

Background:

  • Meropenem, a broad-spectrum antibiotic, is crucial in pediatric intensive care units.
  • Therapeutic drug monitoring (TDM) optimizes meropenem dosing but requires large sample volumes, posing challenges in children.
  • Volumetric absorptive microsampling (VAMS) offers a potential solution for reduced sample volume.

Purpose of the Study:

  • To evaluate the reliability of VAMS for meropenem TDM in pediatric patients.
  • To develop and validate a method for meropenem quantification using minimal plasma volume.

Main Methods:

  • VAMS using 10 µL of whole blood (WB) was compared to traditional EDTA-plasma sampling.
  • Meropenem concentrations were quantified using high-performance liquid chromatography with UV detection.
  • Simultaneous samples were collected from critically ill children.

Main Results:

  • No consistent factor was found to reliably calculate meropenem plasma concentrations from WB using VAMS.
  • VAMS proved unreliable for meropenem TDM in this pediatric population.
  • A validated method for quantifying meropenem from 50 µL of plasma was developed with a 1 mg/L limit of quantification.

Conclusions:

  • VAMS using WB is unsuitable for meropenem TDM in pediatric patients.
  • A simple, reliable, and low-cost HPLC-UV method for quantifying meropenem in 50 µL of plasma was successfully established.
  • This new method facilitates effective TDM in children by minimizing sample volume requirements.