Related Experiment Video
Updated: Jul 30, 2025
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
Development of gelatin-coated nanoliposomes loaded with β-cyclodextrin/vitamin D3 inclusion complex for nutritional
Alireza Ebrahimi1, Hamed Hamishehkar2, Sajed Amjadi3
1Student Research Committee and Biotechnology Research Center, Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract:
The aim of this study was to synthesize of β-cyclodextrin/Vitamin D3 (βCD/VitD3) inclusion complex and encapsulation of this complex with gelatin-coated nanoliposomes (NLPs). Fourier transform infrared spectroscopy confirmed the formation of βCD/VitD3 inclusion complex. In the next step, different gelatin concentrations (1, 2, and 4 mg/mL) were used to surface coat the blank NLPs. The concentration of 2 mg/mL of gelatin was chosen as the optimal concentration for coating the complex-loaded NLPs by considering particle size, morphology, and zeta potential. The particle size and zeta potential of the coated complex-loaded NLPs were 117 ± 2.55 nm and 19.8 ± 1.25 mV, respectively. The images taken by transmission electron microscopy confirmed the formation of a biopolymer layer of gelatin around the NLPs' vesicles. The complex encapsulation efficiency inside the NLPs was 81.09%. The βCD/VitD3 complex loaded NLPs and its coated form exhibited a controlled release profile in simulated gastrointestinal condition.
