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Related Concept Videos

CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jul 30, 2025

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
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CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

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CRISPR for neuroscientists.

Georgios Kalamakis1, Randall J Platt2

  • 1Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland; Novartis Institutes for BioMedical Research, 4056 Basel, Switzerland.

Neuron
|May 18, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas technology offers precise genome engineering for health and disease research. This revolutionary tool enables precise control over biological systems, advancing biomedical and neuroscience applications.

Keywords:
CRISPRCRISPR applicationsneuroscience

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Area of Science:

  • Biomedical Sciences
  • Genetics
  • Neuroscience

Background:

  • CRISPR-Cas systems are microbial defense mechanisms repurposed for genome engineering.
  • These systems provide precise control over nucleic acid manipulation and cellular processes.

Purpose of the Study:

  • To describe the development and applications of CRISPR technologies.
  • To highlight limitations and opportunities in CRISPR-based genome engineering.

Main Methods:

  • Leveraging CRISPR-Cas for genome engineering across diverse biological systems.
  • Application in neuroscience for transgenic models, disease modeling, and therapy testing.

Main Results:

  • CRISPR technologies have revolutionized biomedical sciences and neuroscience research.
  • Applications include engineering animal models, disease correction, and cell state programming.

Conclusions:

  • CRISPR offers powerful strategies for understanding and treating diseases.
  • Ongoing research focuses on expanding CRISPR applications and addressing limitations.