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Related Experiment Videos

Consequences of polyspermy in man.

R R Angell, A A Templeton, I E Messinis

    Cytogenetics and Cell Genetics
    |January 1, 1986
    PubMed
    Summary

    Polyspermy, or fertilization by multiple sperm, occurs in 12% of in vitro fertilization cycles. Resulting embryos often show normal development but can have abnormal chromosome numbers due to errors in the first mitotic division.

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    BioMed research international·2014

    Area of Science:

    • Reproductive biology
    • Human embryology
    • In vitro fertilization (IVF)

    Background:

    • Polyspermy, fertilization by more than one sperm, is a known complication in assisted reproductive technologies.
    • Current hormonal regimens for in vitro fertilization (IVF) and embryo transfer are associated with a polyspermy rate of approximately 12%.
    • Oocytes fertilized by two sperm (dispermic oocytes) often exhibit normal cleavage and are morphologically similar to normally fertilized embryos.

    Purpose of the Study:

    • To investigate the chromosomal and DNA content of dispermic embryos resulting from IVF.
    • To determine the origin of chromosomal abnormalities observed in embryos derived from polyspermic fertilization.
    • To elucidate the mechanisms underlying abnormal embryonic development following polyspermic fertilization in IVF.

    Main Methods:

    • Analysis of oocytes and embryos from IVF procedures.
    • Chromosome studies on dispermic embryos to assess ploidy.
    • DNA measurements to quantify genetic content in dispermic embryos.

    Main Results:

    • Approximately 50% of dispermic embryos were found to be triploid.
    • The remaining dispermic embryos exhibited either a normal diploid chromosome complement or a severely depleted set of chromosomes.
    • Dispermic embryos were morphologically indistinguishable from normally fertilized embryos during early cleavage stages.

    Conclusions:

    • Chromosomal abnormalities in dispermic embryos likely arise during the first mitotic division after fertilization.
    • Aberrant spindle formation is implicated as a potential cause of these errors.
    • Understanding these early developmental errors is crucial for improving IVF success rates and embryo selection.

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