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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
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Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
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Ultracentrifugation for Coagulation Testing.

Robert C Gosselin1

  • 1Davis Health System, Thrombosis & Hemostasis Center, University of California, Sacramento, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2023
PubMed
Summary
This summary is machine-generated.

Lipemia, a condition affecting blood plasma, can interfere with coagulation tests. Ultracentrifugation is presented as an effective method to remove lipemia, ensuring accurate coagulation test results.

Keywords:
CoagulationHemostasisLipemiaTestingUltracentrifugation

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Area of Science:

  • Clinical Chemistry
  • Hematology

Background:

  • Lipemia can interfere with the accuracy of various coagulation tests.
  • Modern analyzers can detect hemolysis, icterus, and lipemia (HIL).
  • Interference from lipemia necessitates mitigation strategies for reliable testing.

Purpose of the Study:

  • To describe a method for mitigating lipemia interference in coagulation testing.
  • To provide a detailed ultracentrifugation technique for sample preparation.

Main Methods:

  • Ultracentrifugation was employed to remove lipemic substances from plasma samples.
  • The method focuses on preparing samples for accurate light-based or chronometric coagulation assays.

Main Results:

  • Ultracentrifugation effectively removes lipemia from blood samples.
  • This process allows for more accurate measurements in coagulation testing.

Conclusions:

  • Ultracentrifugation is a viable method to overcome lipemia interference in coagulation diagnostics.
  • Accurate coagulation testing is achievable with proper sample preparation techniques.