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Updated: Jul 29, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
Published on: May 9, 2025
Christopher M Bourne1,2, Patrick Wallisch2,3, Megan Dacek2,3
1Immunology and Microbial Pathogenesis Program, Weill Cornell Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA 10065.
Synthetic Enzyme-Armed KillER (SEAKER) cells, engineered T cells that activate prodrugs, show efficacy against solid tumors in immunocompetent hosts. This adaptable platform overcomes immune barriers, advancing adoptive cell therapy for cancer treatment.
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