Protocol to evaluate the impact of murine MCT1-deficient CD8⁺ T cells on adipogenesis

The infiltration of activated T cells, such as CD8⁺ effector, in metabolic tissues plays a crucial role for the initiation and propagation of obesity-induced inflammation. Given the pivotal role of lactate transporter monocarboxylate transporter 1 (MCT1) in immune cell activation, we present a protocol for the isolation and activation of CD8⁺ T lymphocytes selectively lacking MCT1. We describe steps for the induction of adipocyte differentiation, CD8⁺ T isolation and activation, and adipocyte-CD8⁺ T cell co-culture. We then detail qPCR analysis on differentiated adipocytes. For complete details on the use and execution of this protocol, please refer to Macchi et al.¹.