Circular RNA hsa_circ_0084054 promotes the progression of periodontitis with diabetes via the miR-508-3p/PTEN axis

Related Experiment Video

Updated: Jul 29, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.5K

Linlin He1,2, Chengcheng Liu1,2, Yiying Liu1,2

  • 1State Key Laboratory of Oral Diseases, & National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.

Journal of Periodontal Research
|May 22, 2023
PubMed
Abstract

Insights

Circular RNA hsa_circ_0084054 exacerbates inflammation and oxidative stress in periodontitis with diabetes. It targets miR-508-3p to upregulate PTEN, promoting disease progression and potentially serving as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Diabetes mellitus is a significant risk factor for periodontitis.
  • Circular RNAs (circRNAs) are implicated in inflammatory processes and disease progression.
  • The role of specific circRNAs, like hsa_circ_0084054, in the context of diabetes-associated periodontitis requires elucidation.

Purpose of the Study:

  • To investigate the role and underlying mechanism of the hsa_circ_0084054/miR-508-3p/PTEN axis in periodontitis progression associated with diabetes.
  • To identify potential molecular targets for therapeutic intervention.

Main Methods:

  • circRNA sequencing identified differentially expressed circRNAs in periodontal ligament cells (PDLCs) under high glucose and lipopolysaccharide (LPS) conditions.
  • In vitro and in vivo validation of hsa_circ_0084054 expression.
  • Bioinformatics analysis, dual luciferase reporter assays, and RIP assays elucidated the interaction network.
  • Assessment of cellular inflammation, oxidative stress, and apoptosis markers in PDLCs.

Main Results:

  • hsa_circ_0084054 was significantly upregulated in high glucose + LPS treated PDLCs and in PDL tissues from diabetic periodontitis patients.
  • Silencing hsa_circ_0084054 reduced inflammatory factors (IL-1β, IL-6, TNF-α), oxidative stress markers (ROS, MDA), and apoptosis, while enhancing SOD activity.
  • hsa_circ_0084054 acts as a sponge for miR-508-3p, leading to PTEN upregulation, inhibited AKT phosphorylation, and aggravated inflammation and oxidative stress.

Conclusions:

  • hsa_circ_0084054 plays a critical role in exacerbating inflammation and promoting the progression of periodontitis in diabetic patients.
  • The hsa_circ_0084054/miR-508-3p/PTEN signaling axis is a key mechanism driving disease pathogenesis.
  • hsa_circ_0084054 represents a promising novel therapeutic target for periodontitis associated with diabetes.
Keywords:
PTENhsa_circ_0084054miR-508-3pperiodontitis with diabetes

More Related Videos

Inducing Apical Periodontitis in Mice10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

12.2K
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model06:14

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

4.1K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K