Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cluster Sampling Method01:20

Cluster Sampling Method

12.0K
Appropriate sampling methods ensure that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
To choose a cluster sample, divide the population into clusters (groups) and then randomly select some of the clusters. All the members from these clusters are in the cluster sample. For example, if you randomly sample four departments from your...
12.0K
Sampling Plans01:23

Sampling Plans

221
Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...
221
Precipitation Processes01:12

Precipitation Processes

495
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
495

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell Fusion in Reprogramming and Regeneration.

Advances in experimental medicine and biology·2026
Same author

A decade of concussion in rugby: a 2014-2024 systematic review and meta-analysis update.

British journal of sports medicine·2026
Same author

Exploring proteomic signatures in sepsis and non-infectious systemic inflammatory response syndrome.

PloS one·2026
Same author

A framework for the exploration of subcellular compartmentalization of RNA-binding proteins.

Nature communications·2026
Same author

Biological protection of grapevine pruning wounds: Training system design and mycobiome dynamics.

Pest management science·2026
Same author

Humanizing medical care for individuals with autism spectrum disorder and their families: the experience of healthcare support in the Comprehensive Medical Care Unit for individuals with ASD (AMITEA).

Frontiers in psychology·2026
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jul 29, 2025

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'
10:31

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Published on: February 10, 2017

11.1K

STORM Microscopy and Cluster Analysis for PcG Studies.

Laura Martin1, Alvaro Castells-Garcia2, Maria Pia Cosma3,4,5,6

  • 1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2023
PubMed
Summary
This summary is machine-generated.

Advanced microscopy using STochastic Optical Reconstruction Microscopy (STORM) visualizes molecular organization in cells. A new clustering algorithm quantifies polycomb group protein distribution in chromatin at nanometric resolution.

Keywords:
AntibodiesChromatinCluster analysisHistone modificationsImagingMicroscopyPcGPolycombSTORMSuper-resolution

More Related Videos

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
13:02

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Published on: October 5, 2016

10.5K
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

7.0K

Related Experiment Videos

Last Updated: Jul 29, 2025

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'
10:31

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Published on: February 10, 2017

11.1K
The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
13:02

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Published on: October 5, 2016

10.5K
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

7.0K

Area of Science:

  • Cell biology
  • Molecular biology
  • Biophysics

Background:

  • Advanced microscopy techniques like STORM, STED, and SIM enable visualization beyond the diffraction limit.
  • This allows unprecedented insight into molecular organization within single cells.

Purpose of the Study:

  • To apply STochastic Optical Reconstruction Microscopy (STORM) for studying polycomb group proteins (PcG) in chromatin organization.
  • To develop and present a clustering algorithm for quantitative analysis of nuclear molecule spatial distribution.

Main Methods:

  • Utilized 2D STORM imaging to visualize nuclear molecules such as EZH2 and H3K27me3.
  • Developed a distance-based clustering algorithm analyzing STORM localization coordinates (x-y).
  • Classified clusters into 'singles' or 'islands' and calculated metrics like localization count, area, and nearest distance.

Main Results:

  • The clustering algorithm quantitatively analyzes the spatial distribution of nuclear molecules.
  • It classifies clusters and provides metrics for detailed spatial analysis.
  • Demonstrated the approach's applicability to various adherent cell lines and available antibodies.

Conclusions:

  • This strategy offers a comprehensive method for visualizing and quantifying PcG protein and histone mark organization.
  • Achieves nanometric resolution for studying nuclear organization.
  • Enables detailed analysis of molecular arrangements within the cell nucleus.