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Updated: Jul 29, 2025
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
7.8K
Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs
Jong-Nam Oh1, Jinsol Jeong1, Mingyun Lee1
1Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Data in Brief
|May 22, 2023
Summary
Extending in vitro embryo culture is crucial for developmental biology. This study successfully cultured blastocysts on feeder cells for 14 days, enabling extended embryo development and analysis.
Area of Science:
- Developmental Biology
- Embryology
- Genomics
Background:
- Embryo development in vivo is limited by uterine absence in vitro.
- Current in vitro embryo culture is restricted to approximately one week.
- Extending embryo culture duration is vital for research.
Purpose of the Study:
- To extend the in vitro culture period for mammalian embryos.
- To establish and characterize cell lines derived from extended embryo cultures.
- To analyze gene expression changes during extended in vitro embryo development.
Main Methods:
- Hatched blastocysts were cultured on feeder cells for 14 days.
- Four distinct cell types were isolated from cultured colonies.
- RNA sequencing (NovaSeq6000) and differential gene expression analysis were performed.
Main Results:
- Successful extension of embryo culture period by 14 days.
- Establishment of four unique cell lines from blastocyst colonies.
- Identification of differentially expressed genes and Gene Ontology terms.
Conclusions:
- Extended in vitro embryo culture is feasible using feeder cell systems.
- The established cell lines provide a model for studying later embryonic development.
- Data offers insights for improving and extending in vitro embryo culture duration.
