Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

6.7K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Echinacoside restores levofloxacin activity against multidrug-resistant Pseudomonas aeruginosa: evidence for iron-responsive siderophore-like properties and membrane disruption.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Decoupling the Effects of Ion Gating from Photoinduced Quasi-Fermi Level Splitting at Photoelectrochemical Interfaces.

ACS applied materials & interfaces·2026
Same author

Selenium-Based Nanoplatforms: An Emerging Theranostic Paradigm for Gynecological Cancers.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

MxDiffusion: A Physics-Aware Maxwell's Law-Guided Diffusion Model Strategy for Inverse Photonic Metasurface Design.

Nano letters·2026
Same author

Endogenous retrovirus-derived RNA-DNA hybrids induce microglial synaptic pruning in autism models.

Neuron·2026
Same author

Identification of an age-related monocyte-derived macrophages characteristic genes-based signature for high-grade diffuse glioma.

Scientific reports·2026

Related Experiment Video

Updated: Jul 29, 2025

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.6K

Limit-Defying μ-Total Analysis System: Achieving Part-Per-Quadrillion Sensitivity on a Hierarchical Optofluidic SERS

Subhavna Juneja1,2, Boxin Zhang1, Alan X Wang1,2

  • 1School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, Oregon 97331, United States.

ACS Omega
|May 22, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces an advanced optofluidic sensor using silver nanoparticle-decorated diatomaceous earth (AgNPs-DE) for highly sensitive fentanyl detection. The novel micro-total analysis system (μ-TAS) achieves part-per-quadrillion sensitivity in minutes.

More Related Videos

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

9.3K
Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

12.9K

Related Experiment Videos

Last Updated: Jul 29, 2025

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.6K
Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

9.3K
Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

12.9K

Area of Science:

  • Optofluidics
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Optofluidic sensors are crucial for developing advanced smart sensor platforms.
  • Existing methods for detecting trace analytes often lack sensitivity and speed.

Purpose of the Study:

  • To integrate a surface-enhanced Raman scattering (SERS) material with a microfluidic device.
  • To develop a micro-total analysis system (μ-TAS) for ultra-sensitive and rapid analyte detection.

Main Methods:

  • Fabrication of a hierarchical structured μ-TAS using silver nanoparticle-decorated diatomaceous earth (AgNPs-DE) on filter paper.
  • Integration of AgNPs-DE with a flow-through microfluidic device.
  • Detection of fentanyl using a portable Raman spectrometer.

Main Results:

  • Achieved part-per-quadrillion (ppq) level sensitivity for fentanyl detection.
  • Demonstrated an ultrafast time of sensing (TOS) of 3 minutes for 10 ppq fentanyl in 10 mL.
  • Reduced the limit of detection-time of sensing (LOD-TOS) product by 4 orders of magnitude compared to traditional methods.

Conclusions:

  • The developed μ-TAS offers unprecedented detection sensitivity and speed for trace analytes.
  • This technology represents a significant advancement in ultrafast sensing for extremely low concentration analytes.
  • The synergistic integration of microfluidics and SERS materials enhances mass-transfer efficiency and detection capabilities.