Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

79
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
79
CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for direct in vivo evaluation of the stretch reflex in adult mice.

STAR protocols·2026
Same author

Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate Ciona robusta.

Differentiation; research in biological diversity·2026
Same author

Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate <i>Ciona robusta</i>.

bioRxiv : the preprint server for biology·2026
Same author

A Tribute to Mark A. Johnson.

The journal of physical chemistry. A·2026
Same author

Robotic mini- or less open sublay for anterior abdominal wall hernia repair using the da Vinci single port robotic platform (SP MILOS): a preclinical feasibility study.

Hernia : the journal of hernias and abdominal wall surgery·2026
Same author

A protocadherin mediates oral placode morphogenesis in the tunicate Ciona.

Biology open·2026

Related Experiment Video

Updated: Jul 29, 2025

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.5K

Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.

Christopher J Johnson1, Akhil Kulkarni1, William J Buxton1

  • 1Georgia Institute of Technology.

Biorxiv : the Preprint Server for Biology
|May 22, 2023
PubMed
Summary

Researchers screened genes in tunicates using CRISPR/Cas9 to understand metamorphosis. They identified Vamp1/2/3 as crucial for sensory papillae to trigger this developmental transition.

More Related Videos

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
08:41

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Published on: May 12, 2018

18.2K
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
09:39

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

9.9K

Related Experiment Videos

Last Updated: Jul 29, 2025

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.5K
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
08:41

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Published on: May 12, 2018

18.2K
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
09:39

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

9.9K

Area of Science:

  • Marine Biology
  • Developmental Biology
  • Neuroscience

Background:

  • Tunicates, sister group to vertebrates, exhibit a biphasic life cycle with distinct larval and adult stages.
  • Understanding tunicate larval settlement and metamorphosis is key to vertebrate evolutionary studies.
  • Sensory neurons in tunicate larvae are implicated in substrate detection and metamorphosis initiation.

Conclusions:

  • The *Vamp1/2/3* gene plays a vital role in tunicate larval metamorphosis.
  • CRISPR/Cas9 technology can be effectively utilized in undergraduate courses for genetic screens.
  • This research contributes to understanding the neural basis of metamorphosis in chordates.