Light-driven ammonium oxidation to dinitrogen gas by self-photosensitized biohybrid anammox systems

The anaerobic ammonium oxidation (anammox) process exerts a very vital role in the global nitrogen cycle (estimated to contribute 30%-50% N₂ production in the oceans) and presents superiority in water/wastewater nitrogen removal performance. Until now, anammox bacteria can convert ammonium (NH₄⁺) to dinitrogen gas (N₂) with nitrite (NO₂^-), nitric oxide (NO), and even electrode (anode) as electron acceptors. However, it is still unclear whether anammox bacteria could utilize photoexcited holes as electron acceptors to directly oxide NH₄⁺ to N₂. Here, we constructed an anammox-cadmium sulfide nanoparticles (CdS NPs) biohybrid system. The photoinduced holes from the CdS NPs could be utilized by anammox bacteria to oxidize NH₄⁺ to N₂. ¹⁵N-isotope labeling experiments demonstrated that NH₂OH instead of NO was the real intermediate. Metatranscriptomics data further proved a similar pathway for NH₄⁺ conversion with anodes as electron acceptors. This study provides a promising and energy-efficient alternative for nitrogen removal from water/wastewater.