Related Experiment Video
Updated: Jul 29, 2025

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
Published on: May 10, 2020
The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells
Poh Kuan Wong1, Nurul Nadia Mohamad Zamberi2, Saiful Effendi Syafruddin2
1Centre for Drug and Herbal Development, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Abstract:
Stem cells such as induced pluripotent stem cells, embryonic stem cells, and hematopoietic stem and progenitor cells are growing in importance in disease modeling and regenerative medicine. The applications of CRISPR-based gene editing to create a mélange of disease and nondisease stem cell lines have further enhanced the utility of this innately versatile group of cells in the studies of human genetic disorders. Precise base edits can be achieved using a variety of CRISPR-centric approaches, particularly homology-directed repair and the recently developed base editors and prime editors. Despite its much-touted potential, editing single DNA bases is technically challenging. In this review, we discuss the strategies for achieving exact base edits in the creation of various stem cell-based models for use in elucidating disease mechanisms and assessing drug efficacy, and the unique characteristics of stem cells that warrant special considerations.
Related Concept Videos
CRISPR
CRISPR/Cas9 Genome Editing
What is Genetic Engineering?
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Homologous Recombination

