Encapsulation of MSCs in PRP-Derived Fibrin Microbeads

Platelet-rich plasma (PRP) is a highly concentrated platelet-containing blood plasma that incorporates a significant amount of growth factors and cytokines needed to accelerate the tissue repair process. PRP has been used effectively for many years in the treatment of various wounds by direct injection into the target tissue or impregnation with scaffold or graft materials. Since autologous PRP can be obtained by simple centrifugation, it is an attractive and inexpensive product for use in repairing damaged soft tissues. Cell-based regenerative approaches, which draw attention in the treatment of tissue and organ injuries, are based on the principle of delivering stem cells to damaged sites by various means, including encapsulation. Current biopolymers used in cell encapsulation have some advantages with some limitations. By adjusting its physicochemical properties, PRP-derived fibrin can become an efficient matrix material for encapsulating stem cells. This chapter covers the fabrication protocol of PRP-derived fibrin microbeads and their use to encapsulate stem cells as a general bioengineering platform for prospective regenerative medical applications.