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Related Experiment Video

Updated: Jul 29, 2025

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Published on: December 22, 2017

Spatial single cell metabolomics: Current challenges and future developments.

Kyle D G Saunders¹, Holly-May Lewis¹, Dany Jv Beste²

¹Department of Chemistry, University of Surrey, Guildford, UK.

Current Opinion in Chemical Biology

|May 24, 2023

Summary

This summary is machine-generated.

Single cell metabolomics offers detailed cellular insights. Combining mass spectrometry imaging and selective cell sampling can overcome current field challenges, advancing this bio-analytical chemistry area.

Keywords:

Lipidomics Mass spectrometry Mass spectrometry imaging Selective cell sampling Single cell metabolomics

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Last Updated: Jul 29, 2025

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Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

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Published on: June 21, 2019

Area of Science:

Bio-analytical chemistry
Cellular biology
Metabolomics

Background:

Single cell metabolomics provides high-resolution cellular analysis.
Current methods include mass spectrometry imaging and selective cell sampling.
Recent advances show potential in observing cell interactions and states.

Purpose of the Study:

To address universal challenges in single cell metabolomics.
To explore complementary strengths of mass spectrometry imaging and selective cell sampling.
To propose collaborative solutions for field advancement.

Main Methods:

Utilizing mass spectrometry imaging for spatial metabolomic data.
Employing selective cell sampling techniques, such as nanocapillary methods.
Analyzing cellular lipids and phenotypes at the single-cell level.

Main Results:

Demonstrated efficacy in observing cell-cell interactions.
Identified lipids crucial for determining cell states.
Achieved rapid phenotypic identification in single cells.

Conclusions:

Single cell metabolomics requires standardized quantification and improved sensitivity/specificity.
Mass spectrometry imaging and selective cell sampling possess complementary advantages.
Collaboration between these two methodological communities can overcome existing limitations.