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Updated: Jul 29, 2025

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

Magnetic-activated cell sorting identifies a unique lung microbiome community.

Daniel G Dunlap^1,2, Libing Yang^3,4, Shulin Qin^3,4

¹Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh School of Medicine and University of Pittsburgh Medical Center, NW628, 3459 Fifth Avenue, Pittsburgh, PA, 15213, USA. dunlapd2@upmc.edu.

|May 24, 2023

Summary

This summary is machine-generated.

Researchers used magnetic-activated cell sorting to identify immunoglobulin G-bound lung bacteria. This method revealed distinct bacterial communities and differences in HIV-positive individuals, linking immune responses to lung bacteria.

Area of Science:

Microbiology
Immunology
Genomics

Background:

Keywords:

Host response Immunoglobulin Microbiome

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Next-generation sequencing reveals distinct lung bacterial communities, though differences between health and disease are often subtle.

Host immune responses may differentiate similar bacterial communities in various populations.

Magnetic-activated cell sorting (MACS) is adapted from gut microbiome studies to analyze immunoglobulin-bound lung bacteria.