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Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays.

Thor Pedersen1,2, Peter Fojan1, Anne Kathrine Nissen Pedersen3

  • 1Department of Materials and Production, Aalborg University, Skjernvej 4A, 9220 Aalborg, Denmark.

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|May 26, 2023
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Summary

This study developed a novel electrochemical immunosensor for rapid biomarker detection at the Point-of-Care. The robust sensor accurately detects Neutrophil Gelatinase-Associated Lipocalin (NGAL) in urine, offering a portable and cost-effective diagnostic solution.

Keywords:
biosensorelectrochemical sensingimmunoassayscreen printed electrodesurface characteristics

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Area of Science:

  • Biosensing and electrochemical detection
  • Biomarker analysis for diagnostics
  • Point-of-Care (PoC) technologies

Background:

  • Current clinical immunoassay methods require specialized equipment, limiting Point-of-Care (PoC) applications.
  • Electrochemical biosensors offer a portable, cost-efficient alternative for analyzing biomarkers in biological fluids.
  • Optimizing sensing surfaces, immobilization, and reporter systems is crucial for enhanced biosensor performance.

Purpose of the Study:

  • To develop and validate a robust electrochemical immunosensor for biomarker detection.
  • To adapt enzyme-linked immunosorbent assay (ELISA) for electrochemical sensing platforms.
  • To evaluate the sensor's performance for detecting Neutrophil Gelatinase-Associated Lipocalin (NGAL) in urine.

Main Methods:

  • Surface characterization of screen-printed and thin-film electrodes using SEM and AFM.
  • Adaptation of ELISA for an electrochemical sensor format.
  • Detection of NGAL in urine to assess sensor robustness and reproducibility.

Main Results:

  • The developed electrochemical immunosensor demonstrated a low detection limit of 1 ng/mL for NGAL.
  • A linear detection range of 3.5-80 ng/mL was achieved with a coefficient of variation (CV%) of 8%.
  • The platform technology proved suitable for immunoassay-based sensors on various electrode types.

Conclusions:

  • The developed electrochemical immunosensor is a viable technology for PoC biomarker detection.
  • The sensor offers a robust, reproducible, and sensitive method for NGAL quantification in urine.
  • This platform technology is adaptable for developing various immunoassay-based electrochemical sensors.