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Characterization of BoHV-4 ORF45
Luca Russo1, Emanuele Capra2, Valentina Franceschi1
1Dipartimento di Scienze Medico Veterinarie, Università di Parma, Parma, Italy.
Frontiers in Microbiology
|May 26, 2023
Summary
Bovine herpesvirus 4 ORF45 is essential for viral replication and particle association. This phosphoprotein impacts cellular pathways, particularly RSK/ERK signaling, offering new research avenues.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus with conserved genes, including orf45.
- BoHV-4 ORF45's structure and function as a tegument protein remain uncharacterized.
- Understanding BoHV-4 ORF45 is crucial for insights into gammaherpesvirus biology.
Purpose of the Study:
- To characterize the structure, function, and cellular impact of BoHV-4 ORF45.
- To investigate the role of ORF45 in BoHV-4 lytic replication and viral particle formation.
- To explore the effects of BoHV-4 ORF45 on the host cell transcriptome.
Main Methods:
- Generation of an ORF45-null mutant BoHV-4 and its pararevertant.
- Biochemical analysis of BoHV-4 ORF45 (phosphorylation, localization).
- Transcriptomic analysis of BoHV-4-infected cells.
Main Results:
- BoHV-4 ORF45 is a nuclear phosphoprotein structurally related to KSHV ORF45.
- ORF45 is essential for BoHV-4 lytic replication and is incorporated into viral particles.
- BoHV-4 ORF45 significantly alters cellular transcriptome, notably affecting RSK/ERK signaling pathways.
Conclusions:
- BoHV-4 ORF45 shares functional and structural similarities with KSHV ORF45.
- ORF45 plays a critical role in BoHV-4 replication and pathogenesis.
- The unique impact of BoHV-4 ORF45 on cellular transcription warrants further investigation.
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