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Bioplastics

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

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Poly(3-hydroxybutyrate) (PHB) and Polycaprolactone (PCL) Based Blends for Tissue Engineering and Bone Medical

Štěpán Krobot¹, Veronika Melčová¹, Přemysl Menčík¹

¹Institute of Material Chemistry, Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic.

|May 27, 2023

Summary

This study developed poly(3-hydroxybutyrate)-based composite blends for bone tissue engineering, finding that PHB/PCL blends showed promising mechanical properties comparable to human bone and were non-cytotoxic, suitable for 3D printing applications.

Keywords:

3D printing FDM biocompatibility bone tissue engineering poly(3-hydroxybutyrate)polycaprolactone polylactic acid scaffold

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Published on: August 8, 2022

Area of Science:

Biomaterials Science
Polymer Science
Tissue Engineering

Background:

Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer with potential for biomedical applications.
Developing PHB-based composites with enhanced properties is crucial for bone tissue engineering.
Optimizing processing and material composition is key to achieving desired performance.

Purpose of the Study:

To prepare and characterize poly(3-hydroxybutyrate)-based composite blends for bone medical applications and tissue engineering.
To evaluate the thermal, mechanical, surface, and cytotoxic properties of these blends.
To assess their suitability for Fused Deposition Modeling (FDM) 3D printing.

Main Methods:

PHB was blended with poly(lactic acid) (PLA) or polycaprolactone (PCL), plasticized with Syncroflex (SN), and incorporated with tricalcium phosphate (TCP).
Materials were processed into 3D printing filaments and samples prepared via FDM 3D printing or compression molding.
Thermal properties (DSC), mechanical strength (tensile, flexural, compression), surface properties (contact angle), and cytotoxicity were evaluated.

Main Results:

Optimal 3D printing temperatures were determined for different blend compositions.
PHB/PCL-based blends exhibited mechanical properties (strength ~40 MPa, modulus ~2.5 GPa) comparable to human trabecular bone.
Two of the three tested blends, specifically the PHB/PCL-based ones, demonstrated non-cytotoxic behavior.

Conclusions:

PHB/PCL-SN and PHB/PCL-SN-TCP blends show significant potential for bone tissue engineering applications due to their mechanical properties and biocompatibility.
Further refinement may be needed to ensure full non-cytotoxicity for all PHB-based formulations.
These materials are suitable for FDM 3D printing, enabling the fabrication of complex scaffolds.