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Updated: Jul 29, 2025

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
Published on: January 18, 2018
Three-Dimensional Chromatin Structure of the EBV Genome: A Crucial Factor in Viral Infection
Lisa Beatrice Caruso1, Davide Maestri1,2, Italo Tempera1
1The Wistar Institute, Philadelphia, PA 19104, USA.
Abstract:
Epstein-Barr Virus (EBV) is a human gamma-herpesvirus that is widespread worldwide. To this day, about 200,000 cancer cases per year are attributed to EBV infection. EBV is capable of infecting both B cells and epithelial cells. Upon entry, viral DNA reaches the nucleus and undergoes a process of circularization and chromatinization and establishes a latent lifelong infection in host cells. There are different types of latency all characterized by different expressions of latent viral genes correlated with a different three-dimensional architecture of the viral genome. There are multiple factors involved in the regulation and maintenance of this three-dimensional organization, such as CTCF, PARP1, MYC and Nuclear Lamina, emphasizing its central role in latency maintenance.
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