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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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A rapid protocol for ribosome profiling of low input samples.

Andreas Meindl1, Markus Romberger1, Gerhard Lehmann2

  • 1Regensburg Center for Biochemistry, University of Regensburg, Regensburg, Germany.

Nucleic Acids Research
|May 29, 2023
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Summary

We developed an ultra-rapid ribosome profiling method for low-input samples. This new protocol enables high-quality translation analysis from small amounts of biological material.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Translational Control

Background:

  • Ribosome profiling offers high-resolution insights into cellular translation.
  • Traditional ribosome profiling is complex, time-consuming, and requires large sample inputs, limiting its application.

Purpose of the Study:

  • To develop an ultra-rapid ribosome profiling protocol.
  • To enable high-quality translation analysis from low-input samples.

Main Methods:

  • Developed a streamlined sequencing library preparation protocol.
  • Utilized solid-phase purification of reaction intermediates.
  • Reduced RNA input requirement to as little as 0.1 pmol.

Main Results:

  • Achieved a one-day turnaround for sequencing library preparation.
  • Demonstrated successful ribosome profiling with significantly reduced sample input.
  • Maintained high sensitivity and data quality.

Conclusions:

  • The new protocol significantly simplifies and accelerates ribosome profiling.
  • Enables ribosome profiling analysis on small or limited samples.
  • Expands the applicability and accessibility of ribosome profiling techniques.