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Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
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An NLR integrated domain toolkit to identify plant pathogen effector targets
David Landry1, Isabelle Mila1, Cyrus Raja Rubenstein Sabbagh1
1Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), INRAE, CNRS, Université de Toulouse, Castanet-Tolosan, F-31326, France.
The Plant Journal : for Cell and Molecular Biology
|May 30, 2023
Summary
Researchers created a library of plant immune receptor integrated domains (IDs) to study interactions with pathogen effectors. They identified novel interactions, including one between a soybean NLR protein and a bacterial effector, advancing plant immunity research.
Area of Science:
- Plant immunity
- Molecular biology
- Genetics
Background:
- Plant immune receptors, NOD-like receptors (NLRs), utilize integrated decoy domains (IDs) to detect pathogen effectors and trigger defense responses.
- Understanding these NLR-effector interactions is crucial for developing disease-resistant crops.
Purpose of the Study:
- To construct a comprehensive library of NLR-integrated domains (IDs) from various plant species.
- To screen this library against conserved bacterial type III effectors to identify novel interaction targets.
- To characterize specific NLR-ID-effector interactions and their functional implications in plant immunity.
Main Methods:
- Compilation of integrated domains (IDs) from NLRs across seven plant species, creating a library of 78 plasmid clones with 104 IDs.
- Yeast two-hybrid assays and in planta interaction tests using 32 conserved type III effectors from Ralstonia pseudosolanacearum.
- Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM) to visualize protein-protein interactions in living plant cells.
Main Results:
- Identified three novel interactions between plant NLR IDs (kinase, DUF3542, WRKY) and two unrelated type III effectors (RipAE, PopP2).
- Discovered a specific interaction between the effector PopP2 and an atypical WRKY domain (ID#85) from a soybean NLR gene (GmNLR-ID#85) in the plant cell nucleus.
- Demonstrated that GmNLR-ID#85, unlike Arabidopsis RRS1-R, was not acetylated by PopP2 and did not trigger immunity.
Conclusions:
- The generated NLR-ID library provides a valuable resource for discovering effector targets and understanding NLR-effector interactions.
- The identified interactions highlight the diversity of NLR domain functions in plant immunity.
- This work offers potential avenues for engineering enhanced plant immune receptors for disease resistance.
