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Genetic circularity of the Proteus mirabilis linkage map
Abstract:
The T incompatibility group plasmid R394 can mobilize the chromosome of Proteus mirabilis strain PM5006. It transferred relatively large segments, corresponding to at least 20 min on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 X 10(-6) per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R+ and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the P. mirabilis strain PM5006 chromosome.
Insights
The R394 plasmid mobilizes the Proteus mirabilis chromosome, transferring large segments and enabling the creation of a composite circular gene map. This study reveals insights into bacterial chromosome mobilization and gene mapping.
Area of Science:
- Microbiology
- Bacterial Genetics
- Molecular Biology
Background:
- The plasmid R394 belongs to the T incompatibility group.
- Proteus mirabilis strain PM5006 is a key organism for this study.
Purpose of the Study:
- To investigate the ability of plasmid R394 to mobilize the chromosome of Proteus mirabilis.
- To construct a composite circular linkage map of the P. mirabilis PM5006 chromosome.
Main Methods:
- Mobilization of the P. mirabilis chromosome by plasmid R394.
- Analysis of recombination frequencies for selected and unselected markers.
- Assembly of chromosomal loci into a linear array.
- Establishing linkage between chromosomal markers and the D plasmid linkage group.
Main Results:
- Plasmid R394 mobilized large chromosomal segments (at least 20 min on the D plasmid map).
- Recombination frequency was constant (5 X 10(-6) per donor cell), suggesting multiple mobilization sites.
- A composite circular linkage map of the P. mirabilis PM5006 chromosome was established.
Conclusions:
- Plasmid R394 is capable of extensive chromosomal mobilization in P. mirabilis.
- The study successfully constructed a composite circular gene map for P. mirabilis PM5006.
- This work provides a foundation for further genetic studies of P. mirabilis.