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Characterization of phages derived from strains of Rhodococcus australis and R. equii.

R Hiddema, M D Curran, N P Ferreira

    Intervirology
    |January 1, 1985
    PubMed
    Summary

    Two novel phages, A and B, were isolated from Rhodococcus strains. These Styloviridae family phages exhibit distinct host ranges but establish stable lysogeny in Rhodococcus rubropertinctus, without transducing genetic markers.

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    Area of Science:

    • Microbiology
    • Virology
    • Bacteriology

    Background:

    • Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecology.
    • Rhodococcus species are Gram-positive bacteria found in various environments.
    • Understanding phage-host interactions is vital for microbial genetics and biotechnology.

    Purpose of the Study:

    • To isolate and characterize novel bacteriophages from Rhodococcus strains.
    • To investigate the host range, morphology, and genetic properties of these phages.
    • To assess the potential of these phages for transduction.

    Main Methods:

    • Spontaneous phage isolation from Rhodococcus australis CSIR-A201 and R. equii CSIR-A655.
    • Host range determination using various Rhodococcus strains, including R. rubropertinctus ATCC 14352.

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  • Morphological characterization using electron microscopy.
  • DNA restriction enzyme analysis.
  • Transduction assays for prototrophic and antibiotic resistance markers.

    • Main Results:

    • Isolation of two distinct temperate phages, designated A and B.
    • Phages A and B belong to the Siphoviridae family, exhibiting similar morphologies.
    • Phages A and B displayed different host ranges but both infected R. rubropertinctus ATCC 14352.
    • Stable lysogeny was achieved upon phage infection.
    • DNA restriction patterns differed between phages A and B and were distinct from actinophage phi EC.
    • No transduction of prototrophic or antibiotic resistance markers was observed.

    Conclusions:

    • Rhodococcus australis and R. equii harbor distinct temperate phages with unique characteristics.
    • These phages, belonging to the Siphoviridae family, establish stable lysogeny in R. rubropertinctus.
    • The observed genetic differences and lack of transduction indicate limited potential for genetic manipulation using these specific phages.