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Updated: Jul 28, 2025

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Viral Transduction of Mammalian Spermatogonial Stem Cells
Makiko Yamada1, Yanyun Luo1, Marco Seandel2
1Department of Surgery, Weill Cornell Medicine, New York, NY, USA.
Abstract:
Lentiviral vectors have been major tools for genetic manipulation of spermatogonial stem cells (SSCs) in vitro. Adeno-associated viral vectors are promising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.
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