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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: Jul 28, 2025

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.

Sarah K Munyoki¹, Kyle E Orwig²

¹Department of Obstetrics, Gynecology, and Reproductive Sciences, Integrative Systems Biology Graduate Program, Magee-Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 30, 2023

Summary

This summary is machine-generated.

Spermatogonial stem cells (SSCs) are key for sperm production. Research in higher primates faces challenges but advances in isolating and transplanting these cells offer hope for treating male infertility.

Keywords:

Adark Apale Grafting Humans Nonhuman primates Spermatogenesis Spermatogonia Transplantation

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Area of Science:

Reproductive biology and stem cell science.
Investigating mammalian spermatogenesis and stem cell applications.

Background:

Spermatogenesis, the process of sperm production, relies on spermatogonial stem cells (SSCs).
SSCs possess the unique ability to self-renew or differentiate, initiating sperm production.
The therapeutic potential of SSCs for male infertility is significant.

Purpose of the Study:

To address challenges in translating rodent SSC knowledge to higher primates (monkeys and humans).
To review progress in characterizing, isolating, culturing, and transplanting SSCs in higher primates.
To overcome limitations in bioassays for non-human primate and human SSCs.

Main Methods:

Characterization of SSCs in higher primates.
Development of methods for isolating and culturing primate SSCs.
Assessment of SSC transplantation and function in primate models.

Main Results:

Progress has been made in understanding primate SSC biology.
Techniques for handling and propagating primate SSCs are advancing.
Challenges remain in establishing robust in vitro and in vivo assays for primate SSCs.

Conclusions:

Translating rodent SSC research to higher primates requires overcoming species-specific differences in terminology and methodology.
Despite challenges, advancements in characterizing, isolating, culturing, and transplanting primate SSCs are promising.
Further development of accessible bioassays is crucial for the clinical application of primate SSCs in treating male infertility.