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Updated: Jul 28, 2025

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
Published on: December 24, 2017
Identifying Fungal Secondary Metabolites and Their Role in Plant Pathogenesis
Joanna Tannous1, Jesse Labbé2,3, Nancy P Keller4,5
1Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. tannousj@ornl.gov.
Abstract:
Pathogenic fungi are the main infectious agents of plants. Secondary metabolites produced by these fungi, also recognized as natural products, are key mediators of plant-fungal interactions. Knowledge on the biosynthesis of these metabolites, the accessibility to fungal genome sequences, and the development of gene disruption techniques open up opportunities to identify many more of these metabolites both in vitro and in planta. This methodology chapter gives a detailed systematic approach aiming to discover new natural products from phytopathogenic fungi and characterize their role in triggering plant cell death and plant disease. This approach takes advantage of the global regulation of fungal secondary metabolite production by regulatory proteins reported in various fungal species.
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