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Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

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Collagen changes in rabbit conjunctiva after conjunctival crosslinking.

Li-Juan Mo¹, Han-Min Wang¹, Huan-Ming Zhou¹

¹Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164 Lanxi Road, Putuo District, Shanghai 200062, China.

Open Life Sciences

|May 30, 2023

Summary

Conjunctival crosslinking with riboflavin and UVA light increased collagen fibril diameter in rabbits. This safe procedure did not cause ultrastructural damage to conjunctival cells, suggesting potential for increased tissue stiffness.

Keywords:

collagen conjunctiva conjunctival crosslinking rabbit

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Area of Science:

Ophthalmology
Biomaterials Science
Tissue Engineering

Background:

Conjunctival crosslinking is explored to enhance tissue stiffness.
Understanding ultrastructural changes in collagen is crucial for evaluating safety and efficacy.

Purpose of the Study:

To investigate the ultrastructural modifications of collagen fibrils in rabbit conjunctiva following crosslinking with riboflavin and ultraviolet A (UVA) light.
To assess the safety and impact on conjunctival cells and collagen expression.

Main Methods:

Rabbit conjunctiva underwent crosslinking using 0.25% riboflavin and UVA light (45 mW/cm² for 4 min).
Collagen fibril ultrastructure was analyzed via electron microscopy.
Collagen I and III expression levels were determined using immunohistochemical staining.

Main Results:

Collagen fibril diameters increased significantly in the treated group (60-90 nm) compared to controls (30-60 nm).
No ultrastructural alterations were observed in conjunctival cells, indicating safety.
While collagen I and III expression increased, statistical significance was not reached for average densities.

Conclusions:

Conjunctival crosslinking with riboflavin and UVA light at 45 mW/cm² for 4 minutes is safe for rabbit conjunctiva.
The procedure leads to an increase in collagen fibril diameter, potentially enhancing conjunctival stiffness.
Further research may explore the functional implications of these ultrastructural changes.