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Detection and Separation of DNA and Silver Nanoparticles Using a Solid-State Nanopore
Bo Ding1, Xin-Yi Hong2, Han Yin2
1Department of Obstetrics and Gynecology, School of Medicine, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, China.
ACS Omega
|May 30, 2023
Summary
Large nanopore sensors can now precisely detect DNA and nanoparticles. This advancement in nanopore sensing technology enables accurate nucleic acid detection for medical diagnostics and more.
Area of Science:
- Nanotechnology
- Biosensing
- Molecular diagnostics
Background:
- Nanopore sensors offer single-molecule detection with potential for gene sequencing.
- Challenges exist in nanopore fabrication, including pore size control and defects.
- Current large-diameter nanopore sensors lack sufficient detection accuracy.
Purpose of the Study:
- To develop precise detection methods for large-diameter nanopore sensors.
- To investigate the use of silicon nitride (SiN) nanopore sensors for detecting DNA and silver nanoparticles (NPs).
- To explore NP-assisted detection of target DNA molecules.
Main Methods:
- Utilized silicon nitride (SiN) nanopore sensors.
- Detected DNA molecules and silver nanoparticles (NPs) individually and in combination.
- Analyzed resistive pulses generated during translocation events.
Main Results:
- Large-sized solid-state nanopore sensors successfully identified and discriminated between DNA, NPs, and NP-bound DNA via resistive pulses.
- Silver NPs were found to bind multiple probes and target DNA, creating larger blocking currents than free DNA.
- Demonstrated that large nanopores can distinguish translocation events for target DNA identification.
Conclusions:
- The developed nanopore-sensing platform enables rapid and accurate nucleic acid detection.
- This method overcomes limitations of small-diameter nanopores and low accuracy of large-diameter ones.
- Significant potential applications include medical diagnosis, gene therapy, and virus identification.
