The let-7b-5p, miR-326, and miR-125a-3p are associated with left ventricular systolic dysfunction in post-myocardial...

Area of Science:

• Cardiovascular Biology
• Molecular Medicine
• Epigenetics

Background:

• Acute ST-elevation myocardial infarction (STEMI) can cause adverse cardiac remodeling, leading to left ventricular systolic dysfunction (LVSd) and heart failure.
• MicroRNAs (miRNAs), as epigenetic regulators, are implicated in the pathophysiology of LVSd.

Purpose of the Study:

• To explore the role of specific microRNAs in peripheral blood mononuclear cells (PBMC) of patients who experienced myocardial infarction and developed LVSd.
• To identify potential miRNA biomarkers for LVSd post-STEMI.

Main Methods:

• Analysis of 61 microRNAs in PBMCs from post-STEMI patients with (n=9) and without (n=16) LVSd using RT-qPCR.
• Identification of differentially expressed miRNAs, followed by Principal Component Analysis and logistic regression for LVSd prediction.
• System biology approach and enrichment analysis to explore miRNA-target regulatory networks and associated biological pathways.

Main Results:

• let-7b-5p, miR-125a-3p, and miR-326 were significantly upregulated in LVSd patients compared to non-LVSd controls.
• These three miRNAs effectively discriminated between LVSd and non-LVSd groups (AUC > 0.78).
• let-7b-5p and miR-326 were identified as independent predictors of LVSd (p < 0.05).
• Enrichment analysis indicated that the targets of these miRNAs are involved in immunological response, cell-cell adhesion, and cardiac remodeling.

Conclusions:

• Left ventricular systolic dysfunction (LVSd) post-STEMI is associated with altered expression of let-7b-5p, miR-326, and miR-125a-3p in PBMCs.
• These miRNAs show potential as diagnostic and predictive biomarkers for LVSd following myocardial infarction.
• The findings suggest a role for these miRNAs in the physiopathology of cardiac dysfunction after STEMI.