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Human Blastocyst Biopsy and Vitrification

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

Bulls fed a high-gain diet decrease blastocyst formation after in vitro fertilization.

Zachary K Seekford¹, Dylan B Davis², Mackenzie J Dickson¹

¹Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.

Reproduction (Cambridge, England)

|May 30, 2023

Summary

This summary is machine-generated.

Feeding bulls a high-gain diet increases their body fat and reduces their sperm quality, significantly lowering the success rate of blastocyst development after in vitro fertilization and embryo culture.

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Area of Science:

Reproductive biology
Animal science
Spermatozoa research

Background:

Bull nutrition significantly impacts reproductive outcomes, yet high-gain diets' effects on embryo development are understudied.
Overfeeding bulls for rapid growth is common in cattle production, raising concerns about sperm and embryo quality.

Purpose of the Study:

To investigate the impact of a high-gain diet on bull sperm quality and subsequent blastocyst development following in vitro fertilization (IVF).

Main Methods:

Eight mature bulls were fed either a maintenance or high-gain diet for 67 days.
Semen was collected, analyzed for sperm quality, and used for IVF.
Embryo development to the blastocyst stage was assessed.

Main Results:

High-gain bulls exhibited increased body weight, average daily gain, and fat thickness.
Sperm from high-gain bulls showed increased early necrosis and post-thaw acrosome damage.
Semen from high-gain bulls resulted in a reduced blastocyst development rate post-IVF.

Conclusions:

A high-gain diet in bulls increases adiposity and impairs sperm function, negatively affecting blastocyst development.
While sperm morphology and motility were unaffected, high-gain diets reduce the capacity for successful embryo creation.