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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
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Spindle Assembly02:50

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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
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The Mitotic Spindle02:27

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The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
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Related Experiment Video

Updated: Jul 28, 2025

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
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SpindlesTracker: An Automatic and Low-Cost Labeled Workflow for Spindle Analysis.

Zhongzhong Li, Yanze Jian, Jiatong Hu

    IEEE Journal of Biomedical and Health Informatics
    |May 30, 2023
    PubMed
    Summary
    This summary is machine-generated.

    SpindlesTracker offers a low-cost, automated method for analyzing spindle dynamics in cell division using fluorescence microscopy. This tool enhances accuracy in tracking spindle elongation, significantly reducing data labeling costs for researchers studying mitosis.

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    Area of Science:

    • Cell Biology
    • Microscopy
    • Computational Biology

    Background:

    • Quantitative analysis of spindle dynamics during mitosis is crucial for understanding cell division.
    • Existing methods struggle with noisy fluorescence microscopy images and high data labeling costs for machine learning.
    • There is a need for efficient, automated, and cost-effective tools for analyzing spindle dynamics.

    Purpose of the Study:

    • To develop a fully automatic and low-cost workflow for analyzing dynamic spindle mechanisms in time-lapse images.
    • To present SpindlesTracker, a novel system for quantitative analysis of spindle dynamics.
    • To address the limitations of existing deterministic and machine learning approaches in spindle analysis.

    Main Methods:

    • Designed YOLOX-SP network for accurate spindle detection and endpoint localization using box-level supervision.
    • Optimized SORT and MCP algorithms for spindle tracking and skeletonization.
    • Created and utilized a novel, annotated S.pombe dataset for training and evaluation.

    Main Results:

    • SpindlesTracker achieved 84.1% mAP in spindle detection and over 90% accuracy in endpoint detection.
    • The workflow reduced data labeling costs by 60% while maintaining high performance.
    • Tracking accuracy improved by 1.3% and tracking precision by 6.5%, with mean spindle length error within 1 μm.

    Conclusions:

    • SpindlesTracker provides an efficient and accurate solution for analyzing mitotic spindle dynamics from time-lapse fluorescence microscopy.
    • The developed workflow significantly lowers costs and improves performance compared to existing methods.
    • The approach has broad implications for studying cell division and can be adapted for analyzing other filamentous structures.