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Related Experiment Videos

Supernatant hemoglobin determinations after prolonged blood storage.

J E Raftos, I M Stewart, V A Lovric

    Pathology
    |January 1, 1986
    PubMed
    Summary

    Measuring supernatant hemoglobin in stored red blood cells is crucial for quality assurance. The cyanomethemoglobin method is recommended for its simplicity and safety in assessing blood storage strategies.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Clinical Chemistry

    Background:

    • Extracellular hemoglobin measurement is vital for evaluating blood storage strategies and quality assurance.
    • Transfusion practices are shifting towards concentrated red cells in additive solutions, necessitating new measurement methods.
    • Accurate hemoglobin estimation in stored blood units is essential for transfusion safety.

    Purpose of the Study:

    • To develop and validate a safe and efficient method for measuring supernatant hemoglobin in stored red cell concentrates.
    • To compare the cyanomethemoglobin method with Tetramethylbenzidine (TMB) methods for hemoglobin quantification.
    • To recommend a suitable method for routine quality assurance of stored blood products.

    Main Methods:

    • Development of a cyanomethemoglobin-based assay for hemoglobin measurement.

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  • Investigation of Tetramethylbenzidine (TMB) methods for quantifying hemoglobin and its derivatives in supernatants.
  • Comparative analysis of the two methods using 68 stored red cell concentrates.

    • Main Results:

    • Both cyanomethemoglobin and TMB methods quantitatively detected hemoglobin in packed cell supernatants.
    • A strong correlation was observed between the results obtained from the cyanomethemoglobin and TMB methods.
    • The cyanomethemoglobin method demonstrated simplicity and adequate sensitivity.

    Conclusions:

    • The cyanomethemoglobin method is a reliable and safe technique for measuring supernatant hemoglobin in stored red cell concentrates.
    • This method avoids carcinogenic benzidine derivatives, making it preferable for routine use.
    • The cyanomethemoglobin method is recommended for routine quality assurance in blood banking and transfusion services.