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Related Experiment Video

Updated: Jul 28, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack

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Published on: August 15, 2018

Reconfigurable pH-Responsive DNA Origami Lattices.

Sofia Julin¹, Veikko Linko^1,2,3, Mauri A Kostiainen^1,2

¹Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, P.O. Box 16100, 00076 Aalto, Finland.

|May 31, 2023

Summary

Researchers developed a pH-responsive DNA origami lattice that can switch between open and closed states. This advancement enables large-scale, dynamic DNA nanostructures with programmable responses to environmental stimuli.

Keywords:

DNA nanotechnology DNA origami DNA triplex hierarchical self-assembly metal nanoparticles pH control

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Last Updated: Jul 28, 2025

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Folding and Characterization of a Bio-responsive Robot from DNA Origami

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Area of Science:

Nanotechnology
Biophysics
Materials Science

Background:

DNA nanotechnology allows precise fabrication of static and dynamic nanostructures.
Current dynamic DNA nanostructures are typically small, discrete, and device-like.
There is a need for large-scale, collectively behaving higher-order systems with dynamic capabilities.

Purpose of the Study:

To demonstrate a large-scale, 2D, pH-responsive DNA origami-based lattice.
To show the lattice can switch between distinct "open" and "closed" configurations.
To explore the use of electrostatic control for lattice formation and conformational switching.

Main Methods:

Assembly of a 2D DNA origami lattice on a mica substrate.
Incorporation of "pH-latches" into DNA origami units for pH responsiveness.
Utilizing directed polymerization and electrostatic control for lattice formation and switching.

Main Results:

Successfully assembled a large-scale, 2D DNA origami lattice.
Demonstrated reversible switching between "open" and "closed" states triggered by pH changes.
Showcased the formation of pH-responsive 2D gold nanoparticle lattices.

Conclusions:

This work bridges DNA origami templates with large-scale, stimuli-induced dynamic systems.
Electrostatic control over DNA origami unit adhesion and mobility is key for lattice formation and switching.
The developed method offers a pathway for creating sophisticated, dynamic nanostructures.