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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
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Research progress in methods for detecting neutralizing antibodies against SARS-CoV-2
Chunxia Chen1, Jiahui Liang1, Hangzhan Hu2
1Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, Henan University, Kaifeng, 475004, China.
Analytical Biochemistry
|May 31, 2023
Summary
Understanding SARS-CoV-2 neutralizing antibody (NAb) attenuation is crucial for preventing reinfection. This review covers NAb detection methods, aiding vaccine development and SARS-CoV-2 control strategies.
Area of Science:
- Immunology
- Virology
- Public Health
Background:
- The SARS-CoV-2 pandemic necessitates understanding viral immunity.
- Neutralizing antibodies (NAbs) are key to preventing reinfection.
- Accurate NAb detection is vital for vaccine efficacy and public health strategies.
Purpose of the Study:
- To review current technologies for detecting SARS-CoV-2 neutralizing antibodies (NAbs).
- To provide insights into NAb attenuation rules in vivo.
- To support SARS-CoV-2 epidemic prevention and control efforts.
Main Methods:
- Literature review of SARS-CoV-2 NAb detection technologies.
- Analysis of common detection methods including virus neutralization tests, pseudovirus assays, lateral flow assays, and ELISAs.
- Synthesis of information on NAb detection's role in immunity evaluation.
Main Results:
- Multiple methods exist for NAb detection, each with specific applications.
- NAb detection is essential for assessing post-vaccination or post-infection immunity.
- Understanding NAb dynamics informs vaccine iteration and reinfection prevention.
Conclusions:
- Effective SARS-CoV-2 control relies on robust NAb detection methods.
- Further research into NAb attenuation rules is needed.
- This review offers a foundation for advancing SARS-CoV-2 countermeasures.
