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Updated: Jul 28, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Published on: April 30, 2018
New possibilities for trait improvement via mobile CRISPR-RNA
Qamar U Zaman1, Ali Raza2, Rafaqat Ali Gill3
1Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya 572025, China; College of Tropical Crops, Hainan University, Haikou 570228, China.
Abstract:
CRISPR/Cas9 gene technology is transported as RNA from transgenic roots to distal parts of unmodified grafted scion, where it is translated into proteins to induce heritable mutagenesis at desired loci. This technique has the potential to produce transgene-free and genetically stable plants in difficult-to-propagate and near-extinct species.
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