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Updated: Jul 28, 2025
Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
Targeted Quantification of Amino Acids by Dansylation
Yuanyuan Liu1, Haoqing Chen1, Dylan Dodd2,3
1Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
We developed a simple, rapid, and robust method for quantifying amino acids in biological samples using chemical derivatization and liquid chromatography-mass spectrometry (LC-MS). This protocol enables easy analysis of 20 proteinogenic amino acids for metabolic studies.
Area of Science:
- Biochemistry and analytical chemistry.
Background:
- Accurate amino acid quantification is vital for understanding metabolic pathways.
- Existing methods for amino acid analysis present challenges in sample preparation, dynamic range, reproducibility, and throughput.
Purpose of the Study:
- To present a simple, rapid, and robust method for amino acid analysis.
- To provide a detailed protocol for analyzing 20 proteinogenic amino acids in biological samples.
Main Methods:
- Chemical derivatization of amino acids.
- Analysis using liquid chromatography-mass spectrometry (LC-MS).
Main Results:
- The presented method is simple, rapid, and robust.
- A detailed protocol for analyzing 20 proteinogenic amino acids is provided.
Conclusions:
- The developed method facilitates straightforward implementation on modern LC-MS instruments.
- This protocol supports critical research in metabolism through accessible amino acid quantification.
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