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Updated: Jul 28, 2025
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
Published on: April 3, 2014
13C Isotope Labeling and Mass Spectrometric Isotope Enrichment Analysis in Acute Brain Slices
Elisa Motori1,2, Patrick Giavalisco3
1Institute of Biochemistry, University of Cologne, Cologne, Germany. elisa.motori@uni-koeln.de.
Abstract:
Feeding of stable 13C-labeled compounds coupled to mass spectrometric analysis has enabled the characterization of dynamic metabolite partitioning in various experimental conditions. This information is particularly relevant for the study and functional understanding of brain metabolic heterogeneity. We here describe a protocol for the analysis of metabolic enrichment analysis upon feeding of murine acute cerebellar slices with 13C-labeled substrates.
